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  1. What features can be seen in normal skin using RCM?
  2. Reflectance Confocal Microscopy for Skin Diseases
  3. Reflectance Confocal Microscopy for Skin Diseases | SpringerLink
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Thus, migration process imaging provides valuable data regarding accurate function and regulation of leukocyte recruitment to injury site with potential impact in therapeutic purposes 4. Capillary blood flow within dermal vascularization is a parameter that can be easily monitored in real-time, in vivo with RCM imaging due to brightly reflecting of blood cells. Several factors are usually recorded, thus, the quantitative blood cell flow per minute is measured, taking into account the number of capillary loop and multiple fields of view digitally measured for a fixed time-point e.

Further, the capillary loop diameter and the density of dermal capillaries per area are registered in the dermal papillae of the epidermal-dermal junction, where multiple fields of view are captured in real-time images In addition, in real-time investigation of skin blood flow changes induced by topical capsaicin using RCM was proposed as a research model to test neurovascular reactivity 5.

What features can be seen in normal skin using RCM?

An alternative to RCM is two-photon excitation microscopy that provides advantages for 3D and deep tissue imaging. A widespread application of this tool is to evaluate the blood flow via blood cell velocity and brightness, these measurements being very useful in setting different experimental models such as stroke, embryo development 57 or different skin cancers basal cell carcinoma, squamous cell carcinoma where morphological and functional assessments of skin layers are primarily made by combining techniques as laser Doppler flowmetry and RCM Skin reactivity to certain local stimuli could be an excellent model for revising various skin conditions through evaluation of the inflammatory process at the skin level 6 , Neurogenic inflammation was experimentally locally induced by capsaicin and further CLSM in reflectance mode enabled the assessment of the cutaneous micro-vascularization Fig.

This could represent an important research model for studying the link between cutaneous diseases and the nervous system 6. In vivo RCM sequential images 0. In real-time blood cells can be observed as moving bright elements inside the lumina of dermal capillaries. Dilation of dermal capillaries and flow of blood cells inside their lumina can be easily observed after 25 min of topical capsaicin.

As an innovative non-invasive method for in vivo imaging of skin structure, CLSM could be successfully applied in dermatological fields less studied until now in terms of imaging, such as experimental contact dermatitis. Such attempts have been made for almost one decade when in situ imaging of skin reactions produced by sodium lauryl sulphate and pelargonic acid as experimental irritants was reported; the group describes extended cell boundaries, keratinocyte swelling pelargonic acid and induction of parakeratosis within the stratum corneum sodium lauryl sulphate In vivo CLSM is a novel imaging technique that provides the morphological and dynamic characterization of skin structures with a high, quasi-microscopic resolution.

The non-invasive character of the examination and the possibility to evaluate the same skin area at different time-points make in vivo CLSM a useful tool in the diagnosis and monitoring of inflammatory skin diseases.

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Reflectance Confocal Microscopy for Skin Diseases

Moreover, it is an excellent method to study in real-time the dynamic components of skin inflammation, response to treatment or specific stimuli with broad applications ranging from clinical to experimental, functional studies involving the skin. Studying the components of skin inflammation is also of great potential to unravel pathways in the pathogenesis of diseases associated with skin inflammation and might contribute to the development of new treatment strategies.

All authors read and approved the final version of the manuscript. Skin Res Technol. J Med Life. J Invest Dermatol. Acta Dermatovenerol Croat. J Biomed Opt. Dermatol Pract Concept. View Article : Google Scholar. G Ital Dermatol Venereol. In Italian. Exp Dermatol. Oncol Lett. Rom J Morphol Embryol. Mol Clin Oncol. Pol J Pathol. Cytometry B Clin Cytom. J Eur Acad Dermatol Venereol.

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J Med. Br J Dermatol. Arch Dermatol. J Dermatol Case Rep. Cell Biochem Biophys.

Reflectance Confocal Microscopy for Skin Diseases | SpringerLink

Actas Dermosifiliogr. Wound Repair Regen. J Am Acad Dermatol. Rev Rom Bioet.

Reflectance Confocal Microscopy

J Digit Imaging. Microsc Res Tech. Biomarkers Med. N Engl J Med. Lever's Histopathology of the Skin. Levine A, Markowitz O. Introduction to reflectance confocal microscopy and its use in clinical practice. DermNet NZ does not provide an online consultation service. If you have any concerns with your skin or its treatment, see a dermatologist for advice. Advertisement Skin cancer App to facilitate skin self-examination and early detection.


  • Justin y la espada del valor (Spanish Edition)?
  • Reflectance Confocal Microscopy for Inflammatory Skin Diseases.?
  • About this book.
  • Review paper The use of reflectance confocal microscopy in selected inflammatory skin diseases.
  • In Vivo Reflectance Confocal Microscopy | MDedge Dermatology.

Reflectance confocal microscopy for in vivo skin imaging. Photochemistry and photobiology. New directions in dermatopathology In vivo confocal microscopy in clinical practice. Dermatol Clin.

Reflectance confocal microscopy for skin diseases. Heidelberg:Springer; Medicine Dermatology. Free Preview. This unique book encompasses the most relevant and up-to-date aspects of RCM reflectance confocal microscopy It serves as a helpful hands-on guide for confocal imaging for dermatologists It includes a chapter devoted to bridging the gap between dermoscopy, RCM and histopathology All chapters are lavishly illustrated and reader-friendly structured see more benefits. Buy eBook. Buy Hardcover. Buy Softcover. Rent the eBook.

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FAQ Policy. About this book This book focuses on the use and significance of in vivo reflectance confocal microscopy RCM for non-invasive high-resolution imaging of the skin. Show all. Pages Dermoscopic and Histopathologic Correlations Pupelli, Gaia et al. Common Nevi Bassoli, Sara et al.

Spitz Nevi Bassoli, Sara et al. Superficial Spreading Melanoma Longo, Caterina et al.